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KMID : 0363620180390030001
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Journal of Korean Oriental Medicine
2018 Volume.39 No. 3 p.1 ~ p.16
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Screening of 56 Herbal formulas covered by the National Health Insurance Service on Dementia-related Factors
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Lim Hye-Sun
Kim Yu-Jin
Kim Yoon-Ju
Kim Bu-Yeo
Jeong Soo-Jin
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Abstract
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Objectives: The purpose of this study was to investigate the effects of 56 herbal formulae covered by the National Health Insurance Corporation (NHIC) on dementia-related biomarkers and neuronal cell changes.
Methods: The 56 herbal formulae were extracted with 70% ethanol at 100°C for 2 h. The antioxidant properties was measured by radical scavenging assay using ABTS+ radical. The acetylcholinesterase (AChE) activity was tested by Ellman’s assay and amyloid-β (Aβ) aggregation was determined using fluorescence method. To estimate the inhibitory effects of herbal formulae on neuronal cell death and inflammation using HT22 hippocampal cells and BV-2 microglia, respectively.
Results: Among the 56 herbal formulae, Dangguiyukhwangtang, Banhasasimtang, Samhwangsasimtang, Cheongwiesan, Hwangryunhaedoktang, Banhabaekchulchunmatang, Jaeumganghwatang, Cheongseoikgitang, and Hoechunyanggyuksan has a significant inhibitory effects on acetylcholinesterase (AChE) activity. Doinseunggitang and Samhwangsasimtang exerted the effect on the inhibition of amyloid-β (Aβ) aggregation. Additionally, 10 herbal formulae affected AChE and Aβ aggregation revealed antioxidant activity as well as neuroprotective and anti-neuroinflammation effects in neuronal cell lines.
Conclusions: 10 herbal formulae that have been shown to be effective against the major dementia markers have been shown to have antioxidant activity, neuronal cell protection and inhibition of brain inflammation. Further investigation of these herbal formulae will need to be validated in dementia animal models.
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KEYWORD
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Dementia, Acetylcholinesterase, Amyloid-β, Antioxidant, Neuronal cells, Herbal formula
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